Authorised officers, customer service staff, conductors and train controllers will stop work between 3am and 4pm on Friday March 8.Â
It's a major escalation in a long-running dispute between rail operator V/Line and the Rail, Tram and Bus union over a new enterprise agreement.
The union claims it received a "sub standard" offer on Friday, sparking what will be the seventh strike this year.
Branch Secretary Vik Sharma claimed Victoria's public sector wages policy, which caps salary increases at three per cent, is being used to "hold back" V/Line employees.
"Regional workers and their communities deserve to be treated with respect, but instead they are being treated like second class citizens," Mr Sharma said in a statement.
"While the Allan state government continues to play puppet master, V/Line need to get serious at the bargaining table to resolve this dispute."
V/Line chief executive Matt Carrick called on the union to resolve the dispute at the bargaining table, claiming the most recent offer had "improved" benefits.
"The union's action to notify us of further strikes is disappointing and this continued action will disrupt passengers that rely on V/Line services," he said.
Train drivers are not part of the strike.
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